N3-alkyl-2&#39;,5&#39;-0-silylated-3&#39;-spiro-thymidine derivatives

ABSTRACT

Nucleoside analogs possessing anti-HIV activity and having a 3&#39;-spiro moiety and blocking groups at the 2&#39;- and 5&#39;-positions. The preferred species is shown in the structure below: ##STR1## wherein R is alkyl.

The present invention relates to substituted nucleoside derivatives, as well as to their chemical synthesis and to antivirally active pharmaceutical compositions containing said compounds.

Among the most potent and selective anti-HIV (Human Immunodeficiency Virus) agents, which seem to act through inhibition of reverse transcriptase, are the 2', 3'-dideoxynucleosides. 3'-Azido-3'-deoxythymidine (AZT) and 2', 3'dideoxy-inosine have been, so far, the only approved drugs for treatment of AIDS in humans.

It is now well established that AZT prolongs the life of ARC and AIDS patients, decreases the frequency as well as severity of opportunistic infections, and partially restores the immunological competence of the patients. However, long-term treatment of AIDS patients with AZT is compounded by a number of toxic side effects, the main drawback being bone marrow suppression. Shortly after the discovery of AZT as an effective anti-retrovirus agent, various other 2', 3'-dideoxynucleoside (ddN) analogues were identified as potent and selective HIV inhibitors, and some of these ddN analogues are now subject of clinical trials.

Recently, different classes of compounds have been established to inhibit HIV-1, but not HIV-2 or SIV replication. These highly specific HIV-1 inhibitors include the 6-substituted acyclouridine derivatives, tetrahydroimidazoben-zodiazepiones and -thiones, dipyridodiazepinones, pyridinones, and bis(heteroaryl)piperazines.

After extensive research it has now been found that a selective inhibiting effect on HIV-1 is displayed by a novel class of substituted nucleoside derivatives according to claim 1.

The 50% effective concentration (EC₅ O) of the most active of these compounds for HIV-1 ranged from 0.034 to 0.439 μg/ml. The 50% cytotoxic concentration (CC₅ O) for MT-4 cells ranged from 2.35->200 μg/ml.

Initial structure-activity studies revealed that the simultaneous presence of a 3'-spirosubstituent and alkylated silylgroups at the 2' and 5' positions of the ribose results in compounds which are exquisitely inhibitory. Preferred compounds comprise derivatives of formula I wherein B is a N³ -alkylated thymine, the 3'-position of the ribose is substituted with a spiro-substituent and the 2'-and 5'-positions are both substituted with O-silylsubstituents optionally substituted with alkyl or phenyl. The most preferred compounds are {[1-2', 5'-bis-O-(tert-butyldimethylsilyl)-β-D-ribofuranosyl]-3-N-methyl-thymine}-3'-spiro-5"-{4"-amino-1", 2"-oxathiole-2", 2-dioxide} with EC₅ O=0,034 μg/ml, CC₅ O=139 μg/ml and Selectivity Index=4088; or {1-[2', 5'-Bis-O-(tert-butyldimethylsilyl)-β-D-ribofuranosyl]-3-N-ethyl-thymine}-3'-spiro-5"-{4"-amino-1",2"-oxathiole-2", 2"-dioxide} with EC₅ O=0,073 μg/ml, CC₅ O=73 μg/ml and Selectivity Index=1000.

The compounds of this invention are represented by Formulas I and II: ##STR2## wherein:

B represents:

a pyrimidine of the general formula: ##STR3## wherein:

X₅ is a sidechain selected from the group comprising H, alkyl, alkenyl, alkynyl, benzyl, halogen, cyano, thiocyano, --CH═CHR, hydroxymethyl, --CH₂ CH₂ -halogen, nitro, amino;

X₄ is a sidechain selected from the group comprising OH, SH, NH₂, HNCH₃, N(CH₃)₂, NHCOCH₃ ;

X₃ is a sidechain selected from the group comprising H, alkyl, alkenyl, alkynyl, benzyl;

X₂ is a sidechain selected from the group comprising O, S, Se;

or aza- and deaza derivatives thereof;

a purine of the general formula: ##STR4## wherein

X₆ is a sidechain selected from the group comprising H, OH, halogen, NH₂, NHCH₃, N(CH₃)₂ ;

X₇ is a sidechain selected from the group comprising H, alkyl, monomethoxytrityl;

or aza- and deazaderivatives thereof;

a benzimidazole of the general formula: ##STR5## wherein

X₈ are sidechains selected from the group comprising halogen, amino, alkyl, alkenyl, alkynyl, mercapto, optionally substituted;

an imidazole of the general formula: ##STR6## wherein:

X₉ is a sidechain selected from the group comprising H, alkyl, alkenyl, alkynyl, amino, mercapto, optionally substituted,

a triazole of the general formula: ##STR7## wherein

X₁₀ is a sidechain selected from the group comprising H, alkyl, alkenyl, alkynyl, amino, mercapto, optionally substituted;

a sidechain of the formula ##STR8## wherein

R₁ represents a sidechain selected from the group consisting of H, ester, amino acid;

R₃, represents a sidechain of the formula: ##STR9## wherein

R₁ is a sidechain selected from the group comprising H, alkyl, alkenyl, alkynyl, optionally substituted;

or a sidechain of the general formula: ##STR10## wherein

R₁ is a side chain selected from the group comprising H, amino, hydroxyl, carboxyl, mercapto, CH₂ NH₂, CONH₂, CH₂ OH, CH₂ COOH, CH₂ NHOH, CH₂ SH, alkyl, alkenyl, alkynyl, optionally substituted;

R₂ is a sidechain selected from the group comprising H, alkyl, alkenyl, alkynyl, amino, mercapto, optionally substituted;

or a side chain of the general formula: ##STR11## wherein

n≧0, and the ring is either cycloalkyl or phenyl and R₁, R₂, R₃, R₄, R₅ are H, NH₂, SH, CH₂ NH₂, CONH₂, COOH, CH₂ OH, CH₂ NHOH, CH₂ SH, OH, optionally substituted.

or CH₂ NH₂, CONH₂, CH₂ OH, CH₂ NHOH or CH₂ SH;

R₅, and R₂, are the same or different and represent a sidechain selected from the group comprising trimethylsilyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl, dimethylphenylsilyl, alkyl (optionally substituted with amino, hydroxyl, ether) aryl (optionally substituted), (CH₂)_(n) phenyl with n=1-4; or another highly lipophylic moiety;

wherein the sugar is a pentose selected from the group consisting of ribose, arabinose, lyxose, xylose; or a hexose selected from the group consisting of glucose, mannose, galactose.

When HIV-1 strains were selected for resistance against compounds 5, 6 and 7, full resistance of these HIV-1 mutant strains against many other TSAO derivatives was noted but not against TIBO, Nevirapine and Pyridinone. The reverse transcriptase enzyme of the TSAO-resistant HIV-1 strains consistently contained a glutamic acid change at position 138 to lysine. Investigations with this mutated enzyme revealed that it completely lost affinity for TSAO, but not for other HIV-1-specific compounds (i.e. TIBO, Nevirapine, HEPT derivatives). Thus, amino acid-138 (glutamic acid) of HIV-1 RT determines the affinity of TSAO derivatives for HIV-1 RT, as well as the activity of TSAO derivatives against HIV-1 replication.

Based on these findings, it became likely that the TSAO derivatives (by their 4"-amino group of the 3'-spiro substituent) interfere with glutamic acid-138 (by its functional carboxylic acid group). The fact that, in the TSAO-resistant HIV-1 strains, glutamic acid-138 is replaced by lysine, makes it most likely that TSAO molecules containing a --COOH function in the 3'-spiro moiety may become active against TSAO-resistant HIV-1 strains. Also, these data point to the feasibility to replace the 3'-spiro moiety by another chemical entity containing --NH₂, --OH, or --COOH groups to keep the anti-HIV-1 activity of these compounds.

The substituted nucleoside derivatives are preferably synthesized by:

starting with a 3'-keto nucleoside of the formula: ##STR12## wherein B represents a pyrimidine, purine, benzimidazole, imidazole, triazole, all of which may optionally be substituted, and O-silyl can optionally be substituted with alkyl, phenyl or other lipophillic moiety;

replacing the 3'-ketogroup therein by cyanohydrin;

converting said cyanohydrin to cyanomesylate;

converting said cyanomesylate to a spiro group; and

optionally removing one or more protecting silyl groups.

Alternatively, the nucleoside derivatives of formula I can be synthesized by:

using as a starting material a compound of formula: ##STR13##

converting the 3'-keto group therein through a cyanohydrin group to a cyanomesylate group;

converting the isopropylidene dioxy group in the 1,2 -positions to two acetyloxy groups;

replacing the acetyloxy group in the 1- position by a residue of a pyrimidine or purinebase;

converting the cyanomesylate group in the 3'-position to a spiro-group;

silylating one or both hydroxyl groups in the 2' and 5' position; and

optionally deprotecting one of said silylated hydroxy groups.

The invented compounds as well as their chemical synthesis and preparation of the starting materials are illustrated in the following examples, which should not be constructed however, to restrict the invention.

SYNTHESIS EXAMPLE 1 Intermediate Compounds A and B

2', 5'-Bis-O-(tert-butyldimethylsilyl)-3'-C-cyano-3'-O-mesyl-β-D-pentofuranosyl nucleosides.

A mixture of a 3'-ketonucleoside (4 mmol), water (16 mL), ethyl ether (32 mL), sodium bicarbonate (0.64 g, 8 mmol) and sodium cyanide (0.2 g, 4 mmol) was stirred vigorously at room temperature for 16 h. The organic phase was separated, and the aqueous phase was washed with ethyl ether (2×50 mL). The combined ethereal phases were dried over anhydrous sodium sulfate, filtered, and evaporated to dryness. The residue, a mixture of the two epimeric cyanohydrins, was dissolved in dry pyridine (8 mL). To this solution mesyl chloride (1.6 mL, 20 mmol) was added. The mixture was stirred at 8°-10° C. for 48 h, poured into ice and water, and extracted with chloroform (2×50 mL). The combined extracts were washed with 1N-HCl (50 mL) aqueous sodium hydrogencarbonate (50 mL), and brine (50 mL), dried over anhydrous sodium sulfate, filtered and evaporated to dryness. The residue was purified by column chromatography.

Compounds A-1 and B-1

1-[2', 5'-Bis-O-(tert-butyldimethylsilyl)-3'-C-cyano-3'-O-mesyl-β-D-xylofuranosyl]thymine and 1-[2', 5'-Bis-O-(tert-butyldimethylsilyl)-3'-C-cyano-3'-O-mesyl-β-D-ribofuranosyl]thymine.

The residue was chromatographed with ethyl acetate/hexane (1:4) as the eluent. The fastest moving bands afforded 1.18 g (53%) of A-1 a as a white foam: IR (KBr 1375, 1185 cm⁻¹ (SO₂); ¹ H NMR (CDCl₃, 90 MHz) δ 1.96 (s, 3H, CH₃ -5) 3.33 (s, 3H, CH₃ SO₂), 4.05 (m, 2H, H-5'), 4.51 (m, 1H, H-4'), 4.87 (d, 1H, H-2', J_(1'), 2' =2 HZ), 6.03 (d, 1H, H-1'), 7.28 (s, 1H, H-6), 9.12 (bs, 1H, NH-3); ¹³ C NMR (CDCl₃, 20 MHz) δ 12.26 (CH₃ -5), 40.24 (CH₃ SO₂), 59.17 (C-5'), 81.51, 83.55 (C-2', C- 4'), 82.51 (C-3'), 91.57 (C-1'), 111.87, 112.63 (CN, C-5), 134.68 (C-6), 150.22 (C-2), 163.55 (C-4). Anal. (C₂₄ H₄₃ N₃ O₈ SSi₂). C, H, N, S.

The slowest moving bands afforded 0.22 g (10%) of B-1 as a white foam:

IR (KBr) 1375, 1180 cm⁻¹ (SO₂); ¹ H NMR (CDCl₃, 300 MHz) δ 1.94 (s, 3H, CH₃ -5), 3.26 (s, 3H, CH₃ SO₂), 4.04 (m, 2H, H-5', J_(5'a), 5'a =12, J_(4'), 5'b =1.2, J_(4'), 5'b =2.1 Hz), 4.51 (d, 1H, H-2', J_(1'), 2' =8.3 Hz), 4.73 (m, 1H, H-4'), 6.24 (d, 1H, H-1'), 7.38 (s, 1H, H-6), 8.48 (bs, 1H, NH-3); ¹³ C NMR (CDCl₃, 20 MHz) δ 12.05 (CH₃ -5), 40.33 (CH₃ SO₂), 62.09 (C-5'), 80.34 (C-3'), 78.20, 84.10, 84.28 (C-2', C- 1'), 112.24, 114.19 (C-5, CN), 133.68 (C-6), 150.42 (C-2), 163.18 (C-4). Anal. (C₂₄ H₄₃ N₃ O₈ SSi₂). C, H, N, S.

Compounds A-2 and B-2

1-[2', 5'-Bis-O-(tert-butyldimethylsilyl)-3'-C-cyano-3'-O-mesyl-β-D-xylofuranosyl]uracil and 1-[2', 5'-Bis-O-(tert-butyldimethylsilyl)-3'-C-cyano-3'-O-mesyl-β-D-ribofuranosyl]uracil.

The residue was chromatographed with ethyl acetate/hexane (1:4) as the eluent. The fastest moving bands afforded 0.26 g (11%) of B-2 as a white foam: IR (KBr 1375, 1180 cm⁻¹ (SO₂); ¹ H NMR (CDCl₃, 90 MHz) δ 3.23 (s, 3H, CH₃ SO₂) 4.03 (m, 2H, H-5'), 4.56 (d, 1H, H-2'), J_(1'), 2' =7.5 Hz), 4.73 (m, 1H H-4'), 5.76 (dd, 1H, H-5), 6.26 (d, 1H, H-1'), 7.80 (d, 1H, H-6), 8.96 (bs, 1H, NH-3); ¹³ C NMR (CDCl₃, 20 MHz) δ 40.33 (CH₃ SO₂), 62.46 (C-5'), 78.76, 80.50, 84.33, 84.93 (C-2', C-3', C-4', C-1 '), 103.59 (C-5), 114.00 (CN), 138.73 (C-6), 150.27 (C-2), 162.55 (C-4). Anal. (C₂₃ H₄₁ N₃ O₈ SSi₂). C, H, N, S.

From the slowest moving bands A-2 (1.25 g, 52%) was isolated as a white foam:

IR (KBr) 1375, 1185 cm⁻¹ (SO₂); ¹ H NMR (CDCl₃, 90 MHz) δ 3.20 (s, 3H, CH₃ SO₂), 4.03 (m, 2H, H-5'), 4.60 (m, 1H, H-4'), 4.93 (d, 1H, H-2', J_(1'), 2' =1.5 Hz), 5.73 (d, 1H, H-5), 5.86 (d, 1H, H-1'), 7.46 (d, 1H, H-6), 9.73 (bs, 1H, NH-3); ¹³ C NMR (CDCl₃, 20 MHz) δ 40.01 (CH₃ SO₂), 61.88 (C-5'), 77.71, 80.06, 82.51, 86.42 (C-2', C-3', C-4', C-1'), 105.10 (C-5) 114.33 (CN), 139.10 (C-6), 149.69 (C-2), 163.00 (C-4). Anal. (C.sub. 23 H₄₁ N₃ O₈ SSi₂). C, H, N, S.

Compounds A-3 and B-3

4-N-Acetyl-1-[2', 5'-bis-O-(tert-butyldimethylsilyl)-3'-C-cyano-3'-O-mesyl-β-D-xylofuranosyl]cytosine and 4-N-acetyl-1-[2', 5'-bis-O-(tert-butyldimethylsilyl)-3'-C-cyano-3'-O-mesyl-β-D-ribofuranosyl]cytosine.

The residue was chromoatographed with ethyl acetate/hexane (1:1) as the eluent. The fastest moving fractions afforded 1.3 g (53%) of A-3 as a white foam:

IR (KBr) 1375, 1185 cm⁻¹ (SO₂); ¹ H NMR (CDCl₃, 90 MHz) δ 2.28 (s, 3H, NAc), 3.17 (s, 3H, CH₃ SO₂), 4.10 (m, 2H, H-5'), 4.76 (m, 1H, H-4'), 5.17 (d, 1H, H-2'), J_(1'), 2' =1.2 Hz), 5.84 (d, 1H, H-1'), 7.50 (d, 1H, H-5), 7.82 (d, 1H, H-6), 10.6 (bs, 1H, NH-4); ¹³ C NMR (CDCl₃, 20 MHz) δ 40.06 (CH₃ SO₂), 59.11 (C-5'), 80.51 (C-3'), 81.57, 85.30 (C-2', C-4'), 93.29 (C-1'), 96.75 (C-5), 112.78 (CN), 143.20 (C-6), 154.87 (C-2), 163.67 (C-4), 171.45 (4NCO). Anal. (C₂₅ H₄₄ N₄ O₈ SSi₂). C, H, N, S.

The slowest moving fractions gave 0.37 g (15%) of B-3 as a white foam:

IR (KBr) 1375, 1180 (SO₂); ¹ H NMR (CDCl₃, 90 MHz) δ 2.30 (s, 3H, NAc), 3.24 (s, 3H, CH₃ SO₂), 4.04 (m, 2H, H-5'), 4.62 (d, 1H, H-2', J_(1'), 2' =8 Hz), 4.74 (m, 1H, H-4'), 6.42 (d, 1H, H-1'), 7.45 (d, 1H, H-5), 8.23 (d, 1H, H-6), 10.28 (bs, 1H, NH-4); ¹³ C NMR (CDCl₃, 50 MHz) δ 40.36 (CH₃ SO₂), 62.30 (C-5'), 80.70 (C-3'), 80.07, 84.46, 85.86 (C-1', C-2', C-4'), 97.81 (C-5), 114.10 (CN), 143.60 (C-6), 154.96 (C-2), 163.23 (C-4), 171.37 (4-NCO). Anal. (C₂₅ H₄₄ N₄ O₈ SSi₂). C, H, N, S.

SYNTHESIS EXAMPLE 2 General Procedure for the Synthesis of Compounds 5, 11 and 15

The {1-[2', 5'-Bis-O-(tert-butyldimethylsilyl)-β-D-pentofuranosyl]nucleosides}-3'-spiro-5"-{4"-amino-1",2"-oxathiole-2",2"-dioxide} were prepared as follows.

To a solution of the intermediate compounds 3'-C-cyano-3'-O-mesyl pentofuranosylucleoside A or B (1 mmol) in dry acetonitrile (10 mL), either Cs₂ Co₃ (325 mg, 1 mmol) or DBU (0.14 mL, 1 mmol) was added. The mixture was stirred at room temperature for 1-24 h, then filtered and evaporated to dryness. The residue was purified by column chromatography.

Compound 5

{1-[2', 5'-Bis-O-(tert-butyldimethylsilyl)-β-D-ribofuranosyl]thymine}-3'-spiro-5"-{4"-amino-1",2"-oxathiole-2", 2"-dioxide}.

According to the general procedure, intermediate compound B-1 was treated with Cs₂ Co₃ for 24 h. The residue was chromatographed with ethyl acetate/hexane (1:3) to give 0.11 g (85%) of compound 5 as an amorphous solid.

IR (KBr) 3400, 3320 cm⁻¹ (NH₂), 1645 (C═C--N); ¹ H NMR [(CD₃)₂ CO, 300 MHz] δ 1.90 (s, 3H, CH₃ -5), 4.06 (m, 2H, H-5', J_(5'), a, 5'b =12.2 Hz), 4.31 (t, 1H, H-4', J_(4'), 5' =3.6 Hz), 4.67 (d, 1H, H-2', J_(1'), 2' =8.0 Hz), 5.75 (s, 1H, H-3"), 6.00 (d, 1H, H-1'), 6.47 (bs, 2H, NH₂), 7.42 (s, 1H, H-6), 10.32 (bs, 1H, NH-3); ¹³ C NMR [(CD₃)₂ CO, 50 MHz] δ 63.05 (C-5'), 72.12, 85.05, 87.62 (C-2', C-4', C- 3"), 92.25, 92.31 (C-1', C-3'), 112.20 (C-5), 136.22 (C-6), 151.60, 152.28 (C-2, C-4"), 163.72 (C-4). Anal. (C₂₄ H₄₃ N₃ O₈ SSi₂). C, H, N.

Compound 11

{1-[2', 5'-Bis-O-(tert-butyldimethylsilyl)-β-D-ribofuranosyl]uracil}-3'-spiro-5"-{4"-amino-1", 2"-oxathiole-2", 2"-dioxide}.

According to the general procedure, intermediate compound B-2 was treated with Cs₂ Co₃ for 24 h. The residue was chromatographed with ethyl acetate/hexane (1:2)as the eluent to give 0.24 g (39%) of compound 11 as an amorphous solid.

IR (KBr) 3390, 3310, 3190 cm⁻¹ (NH₂, NH), 1645 (C═C--N); ¹ H NMR [(CD₃)₂ CO, 300 MHz] δ 4.08 (m, 2H, H-5', J_(4'), 5'a =3.2, J_(4'), 5'b =2.9, J_(5'a), 5' =12.4 Hz), 4.35 (dd, 1H, H-4'), 4.59 (d, 1H, H-2', J_(1'), 2' =8.1 Hz), 5.79 (s, 1H, H-3"), 5.83 (d, 1H, H-5), 6.08 (d, 1H, H-1'), 6.44 (bs, 2H, NH₂), 7.76 (d, 1H, H-6), 10.30 (bs, 1H, NH-3); ¹³ C NMR [(CD₃)₂ SO, 75 MH] δ 60.20 (C-5'), 74.50 (C-2'), 84.38, 84.58 (C-4', C-3"), 89.50 (C-1'), 92.81 (C-3'), 103.23 (C-5), 139.91 (C-6), 150.92 151.32 (C-2, C-4"), 162.73 (C-4). Anal. (C₂₃ H₄₁ N₃ O₈ SSi₂). C, H, N.

Compound 15

{4-N-Acetyl-1-[2', 5'-bis-O-(tert-butyldimethylsilyl)-β-D-ribofuranosyl]cytosine}-3'-spiro-5"-{4"-amino-1",2"-oxathiole-2",2"-dioxide}.

Intermediate compound B-3 was treated with Cs₂ CO₃ according to the general procedure for 1 h. The residue was chromatographed with chloroform/methanol (50:1) as the eluent to give 0.14 g (70%) of compound 15 as an amorphous solid.

IR (KBr) 3410, 3320 cm⁻¹ (NH₂), 1650 (C═C--N); ¹ H NMR [(CD₃)₂ CO, 300 MHz] δ 2.24 (s, 3H, NAc), 4.07 (m, 2H, H-5'), 4.29 (dd, 1H, H-4', J_(4'), 5'a =3.09, J_(4'), 5'b =4.66 Hz), 4.83 (d, 1H, H-2', J_(1'), 2' =7.33 Hz), 5.71 (s, 1H, H-3"), 5.97 (d, 1H, H-1'), 6.61 (bs, 2H, NH₂), 7.46 (d, 1H, H-5), 8.17 (d, 1H, H-6), 9.86 (bs, 1H, NH-4); ¹³ C NMR [(CD₃)₂ CO, 50 MHz] δ 62.97 (C-5'), 75.94, 85.25 (C-2', C-4 '), 91.12, 91.34, 91.50 (C-3', C-3", C-1'), 97.66 (C-5), 147.13 (C-6), 152.80, 155.80 (C-2', C-4"), 164.08 (C-4), 171.64 (4-NCO). Anal. (C₂₅ H₄₄ N₄ O₈ SSi₂). C, H, N.

SYNTHESIS EXAMPLE 3 General Procedure for the synthesis of Intermediate compounds C, D and E

Compounds 5 and 11 can alternatively be obtained as follows. A benzoyl-isopropylidene nucleoside was treated with sodium cyanide in a two-phase ethyl ether/water system, in the presence of NaHCO₃, to obtain a ribocyanohydrin, which was immediately transformed in a cyanomesylate. Hyrdolysis of the 1,2-O-isopropylidene group with aqueous trifluoroacetic acid, followed by reaction with acetic anhydride-/pyridine afforded a diacetate derivative. These were glycosylated with thymine, uracil and 5-ethyluracil to obtain intermediate compounds C-1, C-2, C-3 respectively. Treatment of these intermediate compounds with Cs₂ CO₃ gave intermediate compounds D-1, D-2 and D-3. Deprotection thereof with saturated methanolic ammonia gave fully deprotected intermediate compounds E-1, E-2 and E-3 which with reaction with tert-butyldimethylsily chloride furnished compounds 5, 11 and 10 respectively.

Compound 5

{1-[2',5'-Bis-O-(tert-butyldimethylsilyl)-β-D-ribofuranosyl-]thymine}-3'-spiro-5"-{4"-amino-1",2"-oxathiole-2",2"-dioxide}.

To a suspension of E-1 (0.20 g, 0.55 mmol) in dry acetonitrile (15 mL), 4-dimenthylaminopyridine (0.34 g, 2.76 mmol) and tert-butyldimethylsilyl chloride (0.414 g, 2.76 mmol), was stirred at room temperature for 24 h. The solvent was evaporated to dryness and the residue, dissolved in ethyl acetate (50 mL), was washed with cold (4° C.) 1N HCl (25 mL), water (50 mL) and brine (50 mL). The organic phase was dried over anhydrous Na₂ SO₄, filtered and evaporated to dryness. The residue was chromatographed with chloroform/acetone (8:1) as the eluent to give 5 (0.24 g, 74%) as an amorphous solid.

Compound 11

{1-[2',5'-Bis-O-(tert-butyldimethylsilyl)-β-D-ribofuranosyl]uracil}-3'-spiro-5"-{4"-amino-1",2"oxathiole-2",2"-dioxide}.

To a solution of the cyanomesylate C-2 (0.98 g, 2.0 mmol) in dry acetonitrile (15 mL), Cs₂ CO₃ (0.65 g, 20 mmol) was added. The mixture was stirred at room temperature for 6 h, then filtered, the filtrate was neutralized with acetic acid, and finally, evaporated to dryness. The residue (The spiro derivative D2) was deprotected with saturated methanolic ammonia (20 mL). After standing at room temperature overnight, the solvent was evaporated to dryness. The residue was dissolved in methanol (2 mL) and then treated with chloroform. The solid (the deprotected nucleoside E2) was filtered and suspended in dry acetonitrile (15 mL) and then, 4-dimethylaminopyridine (0.53 g, 4.4 mmol) and tert-butyldimethylsilyl chloride (0.66 g,, 4.4 mmol) were added, stirred at room temperature for 48 h, and evaporated to dryness. The residue was treated with ethyl acetate (100 mL) and water (50 mL). The organic phase was separated, and the aqueous phase was extracted with ethyl acetate (2×50 mL). The combined organics were successively washed with cold (4° C.) 1N HCl (25 mL), water (50 mL) and brine (50 mL) and, finally, dried over anhydrous Na₂ SO₄, filtered and evaporated to dryness. The residue was chromatographed with chloroform/acetone (8:1) as the eluent to give 11 (0.28 g, 24%) as an amorphous solid.

Compound 10

{1-[2', 5'-Bis-O-(tert-butyldimethylsilyl)-β-D-ribofuranosyl]-5-ethyl-uracil}-3'-spiro-5"-{4"-amino-1",2"-oxathiole-2",2"-dioxide}.

A solution of cyanomesylate C3 (1.0 g, 1.91 mmol) in dry acetonitrile (16 mL) was treated with CsCO₃ (0.62 g, 1.91 mmol) for 4 h at room temperature. A similar treatment to that described for the synthesis of compound 5 afforded a residue that was chromatographed with chloroform/acetone (10:1) to give 0.37 g (32% ) of compound 10 as an amorphous solid.

IR (KBr) 3500, 3420 cm⁻¹ (NH₂), 1650 (C═C--N); ¹ H NMR [(CD₃)₂ SO, 200 MHz] δ 1.05 (t, 3H, CH₃ CH₂ -5), 2.26 (m, 2H, CH₃ CH₂), 3.87 (m, 2H, H-5', J_(5'a), 5'b =11.4, J_(4'), 5'a =4.8, J_(4'), 5'b =7.5 Hz), 4.19 (dd, 1H, H-4'), 4.52 (d, 1H, H-2', J_(1'), 2' =8.4 Hz) 5.73 (s, 1H, H-3"), 5.89 (d, 1H, H-1'), 6.93 (bs, 2H, NH₂), 7.56 (s, 1H, H-6), 11.55 (bs, 1H, NH-3); ¹³ C NMR [(CD₃)₂ CO, 50 MHz] δ 14.17 (CH₃ CH₂), 21.20 (CH₃ CH₂), 62.99 (C-5'), 74.99 (C-2'), 85.07, 88.66 (C-4', C-3"), 91.85 (C-3'), 91.98 (C-1'), 118.20 (C-5), 136.56 (C-6), 151.55, 152.28 (C-2, C-4"), 163.23 (C-4). Anal. (C₂₅ H₄₅ N₃ O₈ SSi₂) C, H, N

SYNTHESIS EXAMPLE 4 Synthesis of compounds 16 and 14

Compounds 5 and 11 were reacted further so as to obtain compounds 16 and 14, respectively.

Compound 16

{1-[2', 5'-Bis-O-(tert-butyldimethylsilyl)-β-D-ribofuranosyl]-5-methyl-cytosine}-3'-spiro-5"-{4"-amino-1",2"-oxathiole-2",2"-dioxide}.

To a solution of compound 5 (0.15 g, 0.25 mmol) in dry pyridine (2 mL), a solution of 1,2,4-triazole (0.069 g, 1.01 mmol) and POCl₃ (0.05 mL, 0.5 mmol) in dry pyridine (5 mL) was added. The resulting solution was stirred at room temperature for 4 h, then chilled on ice, and aqueous ammonia (2 mL) was added. After stirring at room temperature for 30 min, the solvent was evaporated to dryness. The residue was treated with 20 mL of a (1:1) mixture of chloroform:methanol. The solid was filtered and the filtrate, after evaporating to dryness, was purified by column chromatography with chloroform/acetone (1:1) as the eluent to give compound 16 (0.10 g, 67%) as an amorphous solid:

IR (KBr) 3350, 3190 cm⁻¹ (NH₂), 1650 (C═C--N); ¹ H NMR [(CD₃)₂ CO, 300 MHz] δ 2.05 (s, 3H, CH₃ -5), 4.02 (dd, 1H, H-5'a, J_(5'a), 5'b =11.7, J_(4'), 5'a =6.8 Hz), 4.09 (dd, 1H, H-5'b, J_(4'), 5' =2.5 Hz), 4.14 H-4'), 5.09 (d, 1H, H-2', J_(1'), 2' =6.6 Hz), 5.51 (d, 1H, H-1'), 5.60 (s, 1H, H-3"), 6.92 (bs, 2H, NH₂ -4"), 7.64 (s, 1H, H-6), 8.22 (bs, 2H, NH₂ -4); ¹³ C NMR [(CD₃)₂ CO, 50 MHz]δ 13.19 (CH₃ -5) 62.64 (C-5'), 74.38 (C-2'), 84.57, 88.98, 95.19 (C-4', C-3", C-140 ), 89.58 (C-3'), 104.06 (C-5), 138.42 (C-6), 154.63, 156.63 (C-2, C-4"), 167.10 (C-4). Anal. (C₂₄ H₄₄ N₄ O₇ SSi₂) C, H, N.

Compound 14

{1-[2', 5'-Bis-O-(tert-butyldimethylsilyl)-β-D-ribofuranosyl]-cytosine}-3'-spiro-5"-{4"-amino-1",2"-oxathiole-2",2"-dioxide}.

Compound 11 (0.18 g, 0.031 mmol) was reacted with 1,2,4-triazole (0.085 g, 1.24 mmol) and POCl₃ (0.06 mL, 0.62 mmol) for 4 h, and then treated with aqueous ammonia, following a similar procedure to that described for the synthesis of compound 16. The residue was chromatographed with chloroform/ethyl acetate (1:2) to yield 0.15 g (84%) of 14 as an amorphous solid;

IR (KBr) 3410, 3350, 3200 cm⁻¹ (NH₂), 1650 (C═C--N); ¹ H NMR [(CD₃)₂ SO, 300 MHz] δ 3.87 (m, 2H, H-5', J_(4'), 5' =5.3 Hz), 4.15 (t, 1H, H-4'), 4.60 (d, 1H, H-2', J_(1'), 2' =7.8 Hz), 5.73 (s, 1H, H-3"), 5.87 (d, 1H, H-5), 5.90 (d, 1H, H-1'), 6.96 (bs, 2H, NH₂ -4"), 7.42 (bs, 2H, NH₂ -4), 7.74 (d, 1H, H-6). Anal (C₂₃ H₄₂ N₄ O₇ SSi₂) C, H, N.

SYNTHESIS EXAMPLE 5 General Procedure for the Synthesis of Compounds 6, 7, 8, 9 and 13.

(3-N-Alkyl-Nucleosides)-3'-spiro-5"-(4"-amino-1",2"-oxathiole-2",2"-dioxide).

To a solution of the spiro nucleoside 5 or 11 (1 mmol) in acetone (12 mL), K₂ CO₃ (0.5 mmol) and the corresponding alkyl halide (1.1-2.0 mmol), were added. The reaction mixture was refluxed for 3-8 h. After removal of the solvent, the residue was purified by column chromatography. Other reaction parameters were as follows.

Compound 6

{1-[2', 5'-Bis-O-(tert-butyldimethylsilyl)-β-D-ribofuranosyl[-3-N-methyl-thymine}-3'-spiro-5"-{4"-amino-1",2"-oxathiole-2",2"-dioxide}

Compound 5 (0.15 g, 0.25 mmol) and methyl iodide (0.07 mL, 0.5 mmol) reacted according to the general procedure for 4 h. The residue was chromatographed with hexane/ethyl acetate (3:1) to give 6 (0.083 g, 55%) as white foam;

IR (KBr) 3390 cm⁻¹ (NH₂), 1715, 1675 (C═O), 1645 (C═C--N)); ¹ H NMR [(CD₃)₂ CO, 200 MHz] δ 1.95 (s, 3H, CH₃ -5), 3.26 (s, 3H, N--CH₃), 4.09 (m, 2H, H-5', J_(gem) =12.2, J_(4'), 5' =3.5 Hz), 4.34 (t, 1H, H-4'), 4.66 (d, 1H, H-2', J_(1'), 2' =8.1 Hz), 5.76 (s, 1H, H-3"), 6.08 (d, 1H, H-1'), 6.45 (bs, 2H, NH₂), 7.50 (s, 1H, H-6). ¹³ C NMR[(CD₃)₂ CO, 50 MHz] 13.10 (CH₃ -5, CH₃ N), 63.14(C-5'), 75.43 (C-2 '), 85.18, 88.27 (C-4', C-3"), 92.47 (C-1', C-3'), 111.33 (C-5), 134.47 (C-6), 152.17, 152.24 (C-2, C-4"), 163.37 (C-4). Anal. (C₂₅ H₄₅ N₃ O₈ SSi₂) C, H, N.

Compound 7

{1-[2', 5'-Bis-O-(tert-butyldimethylsily)-62 -D-ribofuranosyl]-3-N-ethyl-thymine}-3'-spiro-5"-{4"-amino-1", 2"-oxathiole-2",2"-dioxide}

Compound 5 (0.15 g, 0.25 mmol) and ethyl iodide (0.077 mL, 0.5 mmol) reacted, according to the general procedure, for 8 h. The residue was chromatographed with hexane/ethyl acetate (3:1) to yield 0.12 g (77%) of 7 as an amorphous solid;

IR (KBr) 3400, 3320 cm⁻¹ (NH₂), 1715, 1670 (C═O), 1645 (C═C--N)); ¹ H NMR [(CD₃)₂ CO, 300 MHz] δ 1.15 (t, 3H, N--CH₂ CH₃, J=7.05 Hz), 1.94 (s, 3H, CH₃ -5), 3.95 (q, 2H, N--CH₂ CH₃), 4.11 (m, 2H, H-5', J_(gem) =12.2, J_(4'), 5'a =3.6, J_(4'), 5'b =3.5 Hz), ), 4.34 dd, 1H, H-4'), 4.66 (d, 1H, H-2', J_(1'), 2' =8.15 Hz), 5.77 (s, 1H, H-3"), 6.10 (d, 1H, H-1'), 6.48 (bs, 2H, NH₂), 7.49 (s, 1H, H-6); ¹³ C NMR [ (CD₃)₂ CO, 50 MHz]δ 13.00 (CH₃ -5), CH₃ CH₂ N), 36.98 (CH₃ CH₂ N), 63.14 (C-5'), 75.32 (C-2'), 85.14 (C-4'), 88.00 (C-3"), 92.52 (C-1', C-3'), 111.56 (C-5), 134.56 (C-6), 151.89, 152.20 (C-2, C-4"), 162.98 (C-4). Anal. (C₂₆ H₄₇ N₃ O₈ SSi₂) C, H, N.

Compound 8

{1-[2', 5'-Bis-O-(tert-butyldimethylsilyl)-β-D-ribofuranosyl]-3-N-allyl-thymine}-3'-spiro-5"{4"-amino-1", 2"-oxathiole-2", 2"-dioxide}.

Compound 5 (0.15 g, 0.25 mmol) was reacted with allyl bromide (0.023 mL, 0.27 mmol), according to the general procedure, for 3 h. The residue was chromatographed with chloroform/acetone (10:1) to afford 8 (0.10 g, 65%) as an amorphous solid;

IR (KBr) 3400, 3320 cm⁻¹ (NH₂), 1710, 1670 (C═O), 1645 (C═C--N)); ¹ H NMR [(CD₃)₂ CO, 300 MHz] δ 1.96 (s, 3H, CH₃ -5), 4.10 (m, 2H, H-5', J_(gem) =12.2, J_(4'), 5'a =3.73, J_(4'),5'b =3.56 Hz), 4.35 (dd, 1H, H-4'), 4.52 (d, 2H, N--CH₂ --), 4.70 (d, 1H, H-2'), J_(1'), 2' =8.14 Hz), 5.12-5.25 (m, 2H, CH₂ ═CH), 5.78 (s, 1H, H-3"), 5.86 (m, 1H, CH₂ ═CH), 6.10 (d, 1H, H-1'), 6.49 (bs, 2H, NH₂), 7.54 (s, 1H, H-6); ¹³ C NMR [(CD₃)₂ CO, 50 MHz] δ 13.07 (CH₃ -5), 43.88 (N--CH₂), 63.14 (C-5'). 75.25 (C-2'), 85.17, 88.21 (C-4', C-3"), 92.50 (C-1', C-3'), 111.52 (C-5), 118.04 (CH₂ ═CH), 132.98 (CH₂ ═CH), 134.83 (C-6), 151.78, 152.22 (C-2, C-4"), 162.87 (C-4). Anal. (C₂₇ H₄₇ N₃ O₈ SSi₂) C, H, N.

Compound 9

{1-[2', 5'-Bis-O-(tert-butyldimethylsilyl)-β-D-ribofuranosyl]-3-N-(3-methyl-2-butenyl)-thymine}-3'spiro-5"-{4"-amino-1", 2"-oxathiole-2", 2"-dioxide}.

According to the general procedure, compound 5 (0.15 g, 0.25 mmol) and 4-bromo-2-methyl-2butent (0.93 mL, 1.0 mmol) reacted for 2.5 h. The residue was chromatographed with chloroform/acetone (15:1) to give 9 (0.115 g, 70%) as a white solid. mp 194°-195° C. (chloroform/hexane);

IR (KBr) 3400, 3320 cm⁻¹ (NH₂), 1710, 1675 (C═O), 1645 (C═C--N)); ¹ H NMR [(CD₃)₂ CO, 300 MHz] δ 1.67, 1.79 [2s, 6H, (CH₃)₂ C═C], 1.94 (s, 3H, CH₃ -5), 4.08 (m, 2H, H-5', J_(gem) =12.21, J_(4'), 5'a =3.56, J_(4'), 5'b =3.51 Hz), 4.34 (dd, 1H, H-4'), 4.50 (d, 2H, N--CH₂), 4.66 (d, 1H, H-2', J_(1'), 2' =8.06 Hz), 5.20 (m, 1H, C═CH--CH₂), 5.77 (s, H-3"), 6.10 (d, 1H, H-1'), 6.48 (bs, 2H, NH₂), 7.49 (S, 1H, H-6); ¹³ C NMR [(CD₃)₂ CO, 50 MHz]δ 39.90 (N--CH₂), 63.14 (C-5'), 75.34 (C-2'), 85.14, 88.07 (C-4', C-3"), 92.54 (C-1', C-3'), 111.55 (C-5), 119.06 (C═CH), 134.60 (C-6), 137.16 [(CH₃)₂ C═CH], 151.84, 152.21 (C-2, C-4"), 162.98 (C-4). Anal. (C₂₉ H₅₁ N₃ O₈ SSi₂) C, H, N.

Compound 13

{1-[2', 5'-Bis-O-(tert-butyldimethylsilyl)-62 -D-ribofuranosyl]-3-N-allyl-uracil}-3'-spiro-5"-{4"-amino-1", 2"-oxathiole-2", 2"-dioxide}.

According to the general procedure, compound 11 (0.15 g, 0.26 mmol) reacted with allyl bromide (0.09 mL, 1.04 mmol), for 6 h. The residue was chromatographed with chloroform/acetone (15:1) to give compound 13 (0.143 g, 89%) as an amorphous solid;

IR (KBr) 3400, 3330 cm⁻¹ (NH₂), 1720, 1675 (C═O), 1645 (C═C--N)); ¹ H NMR [(CD₃)₂ CO, 300 MHz] δ 4.07 (m, 2H, H-5', J_(gem) =12.45 Hz), 4.37 (dd, 1H, H-4', J₄, 5a =2.7, J_(4'), 5'b =3.2 Hz), 4.48 (m, 2H, N--CH₂), 4.59 (d, 1H, H-2', J_(1'),2' =8.06 Hz), 5.16 (m, 2H, CH₂ ═CH), 5.80 (s, 1H, H-3"), 5.83 (m, 1H, CH₂ ═CH), 5.94 (d, 1H H-5), 6.15(d, 1H, H-1'), 6.45 (bs, 2H, NH₂), 7.79 (s, 1H, H-6). Anal. (C₂₆ H₄₅ N₃ O₈ SSi₄) C, H, N.

Pharmaceutical compositions comprising compounds of formula I as an active ingredient for treatment of AIDS and/or AIDS-related diseases in humans and animals, might take the form of suspensions, powders, solutions, sprays, emulsions, unguents or creams and can be used for topical administration, intranasal, rectal, vaginal as well as oral or parenteral (intravenous, intradermal, intramuscular, intrathecal etc.) administration. Such compositions can be prepared by combining (e.g. by mixing, dissolving etc.) of the active ingredient of formula I in the form of a free acid or salt with pharmaceutical excipients of a neutral character (such as aqueous or non-aqueous solvents, stabilizers, emulsifiers, detergents, additives) and further if necessary, with dyes and aromatizers. The concentration of the active ingredients in a pharmaceutical composition may vary widely between 0.1% to 100 %, depending on the nature of treatment and mode of administration. Further, the dose of the active ingredients to be administered may vary between 0.1 mg to 100 mg per kg bodyweight.

The anti-HIV properties of the 2', 3', 5'-substituted nucleoside derivates according to the invention are documented by the following examples which should not be read in a restricted sense.

The viruses used were moloney murine sarcoma virus (MSV), HIV-1 (strain HTLV-III_(B), kindly provided by R. C. Gallo and M. Popovic, National Cancer Institute, Bethesda, Md.), HIV-2 (strain LAV-2(ROD)), kindly provided by L. Montagnier (Pasteur Institute, Paris, France), simian immunodeficiency virus (SIV) (strain SIV_(MAC251), kindly provided by C. Bruck, RIT-Smith-Kline, Rixensart, Belgium).

The radiolabeled nucleosides [methyl-³ H]dThd (specific radioactivity: 40 Ci/mmol), [5-³ H]dUrd (specific radioactivity: 27 Ci/mmol), [5-³ H]dCyc (specific radioactivity: 18.2 Ci/mmol) and the radiolabeled amino acid L-[4,5-³ H]leucine (specific radioactivity: 52 Ci/mmol were derived from the Radiochemcial Centre (Amersham, England); [5-³ H]Urd (specific radioactivity: 21 Ci/mmol) and [5-³ H]Cyd (specific radioactivity: 21 Ci/mmol) and were obtained from Moravek Biochemicals Inc. (Brea, Calif.)

EXAMPLE 1 Anti-Retrovirus Activity of Purine and Pyrimidien Nucleoside Analogues.

In order to evaluate the inhibitory effects against retrovirus-induced cytopathogenicity in MT-4cells, said cells (5×10⁵ cells/ml) were suspended in fresh culture medium and infected with HIV-1, HIV-2 or SIOV at 100 times the cell culture infective dose-50 (CCID₅₀) per ml cell suspension (1 CCID₅₀ being the dose infective for 50% of the cell cultures). Then, 100 μl of the infected cell suspension were transferred to microplate wells, mixed with 100 μl of the appropriate dilutions of the test compounds, and further incubated at 37° C. After 5 days, the number of viable cells was determined in a blood cell-counting chamber by trypan blue staining for both virus-infected and mock-infected cell cultures. The 50% effective concentration (EC₅₀) and 50% cytotoxic concentration (CC₅₀)were defined as the compound concentrations required to reduce by 50% the number of viable cells in the virus-infected and mock-infected cell cultures, respectively.

The toxicity of test compounds upon a 2-hr incubation period was determined by exposing MT-4 cells (3.5×10⁵ cells/ml) to 100, 20, 4, 0.8 or 0.16 μg/ml of the test compounds for 2 hours at 37° C. Subsequently the cells were washed with culture medium. The pretreated cells were seeded in microplate wells at 70×10³ cells/200 μl-well and allowed to grow for 3 days at 37° C. Control cultures received similar concentrations of the test compounds during the whole incubation period. Then, cells were counted in a Coulter counter (Harpenden Hertz, England) and the CC₅₀ values were determined for each compound.

The substitutents on the test compounds as well as the results are listed in Table 1.

From the data in Table 1, it is clear that the presence of a 3'-spiro group together with substituted silyl groups at both C-2' and C-5' of the ribose are required for optimal inhibitory activity together with low cytotoxicity, thus resulting in a high selectivity Index (S.I.). It is also obvious that the presence of the silyl groups at C-2' and C-5', but not the 3'-spirosubstitutent are responsible for the cytotoxic activity of the compounds.

The compounds listed in Table 1 that are inhibitory against HIV-1 (HTLV-III_(B)), were also found to be active against the HIV-1/HE strain at the same order of magnitude. None of the test compounds listed in Table 1 proved effective against HIV-2 (strain ROD), SIV (strain MAC₂₅₁) in MT-4 cells or MSV in C3H/3T3 cells at subtoxic concentrations (data not shown). Compounds 9, 12, 14, 16, 17, were also found inactive against another HIV-2 strain (EHO).

EXAMPLE 2 Antiviral and cytotoxic activity of the test compounds upon delayed addition to HIV-1-infected MT-4 cells.

Mt-4 cells were suspended in culture medium, infected with HIV-1 and brought into microplate wells as described above. Then, 100 μl of the appropriate dilutions of the test compounds 5, 6 and 10, as well as the reference compounds AZT and TIBO were added to the HIV-1-infected MT-4 cell cultures at the time of infection (0 hr), or at 8, 16, 24, 40 or 48 hours after infection. After 5 days, the number of viable cells was determined and the EC₅₀ and CC₅₀ values for the test compounds were defined as described in example 1. Delay of addition of the test compounds by 17-24 hours resulted in a 10 -fold decrease of antiviral activity for compounds 5, 6 and 10, and a 2- to 5-fold decrease in activity for AZT and TIBO (data not shown). If the addition of the compounds was delayed by 41 to 48 hours, the antiviral activity of compounds 5, 6 and 10, as well as that of AZT and TIBO, was almost totally lost. However, toxicity of compound 5 for MT-4 cells was not affected by the time that the compound had remained in contact with the cells. In fact, exposure of the MT-4 cells to the test compounds for as short as 24 hours or even 2 hours proved almost as inhibitory to MT-4 cell proliferation as a 1-, 3- or 5-day exposure period. This contrasts with AZT that becomes less cytostatic the shorter its exposure time to MT-4 cells (data not shown).

EXAMPLE 3

Inhibition of the incorporation of radiolabeled DNA, RNA or protein precursors into TCA-insoluble MT-4 cell material.

The incorporation of [methyl-³ H]dThd, [5-³ H]dUrd, [5-³ H]dCyd, [5-³ H]Urd, [5-³ H]Cyd and L-[4,5-³ H]1eu into TCA-insoluble MT-4 cell material was measured in wells of a 96-well microtiter plate (Falcon-3072, Becton-Dickinson, New Jersey). To each well were added 10⁵ MT-4 cells, 0.25 μCi of the radiolabeled precursor and a given amount of test compound. The cells were allowed to proliferate for 20 to 24 hr at 37° C. in a humidified, CO₂ -controlled atmosphere. At the end of this incubation period, the contents of the wells (200 μl) were brought onto 25-mm glass fiber filters (type A/E, Gelman Instrument Company, Ann Arbor, Mich.), and mounted on a Millipore 3025 sampling manifold apparatus. The filters were washed twice with 2 ml and 5 ml cold NaCl/Pi (phosphte-buffered saline), twice with 2 and 5 ml cold 10% trichloroacetic acid (TCA), twice with 5 and 7.5 ml cold 5% TCA, and twice with 1 ml cold ethanol.

The results are shown in Table 2. Most of the most active nucleoside derivatives, including the thymine (5), N³ -methylthymine (6), N³ -allylthymine (8), N³ -dimethylallyl-thymine (9), uracil (11), cytosine (14) and 5-methylcytosine (16) derivatives, that were not cytotoxic at 100 μg/ml to MT-4 cells (Table 1) did not markedly inhibit the incorporation of dThd, dUrd or dCyd into DNA, or incorporation of Urd or Cyd into RNA or incorporation of leucine into protein at a concentration of 100 μg/ml (Table 2). However, those compounds that proved inhibitory to MT-4 cell proliferation (i.e. compounds 5, 11 and 16) were equally inhibitory to the incorporation of the precursors into DNA, RNA or protein (Table 2). Thus, neither the thymine (5) nor the uracil (11) or 5methylcytosine (16) derivative showed preferential inhibition of the incorporation of the precursors (dThd, dUrd, dCyd, Urd, Cyd, leu) over the others.

EXAMPLE 4 Determination of the partition coefficient (P_(a))

Partition of the test compounds between 1-octanol (Merck, Darmstadt, Germany) and 10 mM potassium phosphate buffer, pH, 7.5 (Merck), was measured as described in Balzarini et al., Biochem, Biophys. Res. Commun. 158: 413-422 (1989). Briefly, a 50 μM concentration of the test compound in potassium phosphate buffer was thoroughly mixed with an equal volume of 1-octanol for 30 min at room temperature. Then, the mixture was further equilibrated at room temperature and UV absorption was measured for the aqueous and alcoholic liquid phases. The partition coefficient (P_(a)) was calculated as the ratio of the compound concentration present in the 1-octanol phase to the compound concentration present in the aqueous phase.

Thus the partition coefficients (P_(a)) were determined for a number of test compounds listed in Table 1. 3'-Azido-2',3'-dideoxythymidine (AZT) was included for comparative purpose. The P_(a) values for AZT and compound 1 were 1.12 and 0.117, respectively. Compounds 2, 3, 4, 5, 6, 7, 8, 11, 14, 15 were found to be present in the n-octanol phase by more than 95% compared to the aqueous phase. Consequently, the P_(a) values could not be accurately determined but were invariably higher than 20.

The high P_(a) values of the nucleoside anologues suggest that they might easily cross the blood-brain barrier, a property that should be beneficial in the light of the observations that HIV has marked tropism for the central nervous system.

Due to their lipophilic nature, the test compounds most likely also penetrate into the cells by passive diffusion, rather than by a facilitated transport carrier mechanism.

                                      TABLE 1                                      __________________________________________________________________________     Inhibitory effect against HIV-1 of various nucleoside-derivatives of the       invention.                                                                     Compound                                        CC50 S.I.                      no.   B      X.sub.3                                                                             X.sub.4                                                                             X.sub.5                                                                            X.sub.6                                                                           R.sub.5 '                                                                           R.sub.2 '                                                                           R.sub.3 '                                                                          EC50                                                                               (μl/ml)                                                                          (μl/ml)                __________________________________________________________________________     1     T      --   OH   CH3 -- OH   OH   Spiro                                                                              >100                                                                               >100 --                        2     T      --   OH   CH3 -- OH                                                                                   ##STR14##                                                                          Spiro                                                                              >40 106  <2,5                      3     T      --   OH   CH3 --                                                                                 ##STR15##                                                                          OH   Spiro                                                                              >20 45   <2                        4     T      --   OH   CH3 -- OBZ                                                                                  ##STR16##                                                                          Spiro                                                                              >8  20   <2,5                      5     T      --   OH   CH3 --                                                                                 ##STR17##                                                                           ##STR18##                                                                          Spiro                                                                              0,034                                                                              7,7  227                       6     3-Me--T                                                                               Meth.                                                                               OH   CH3 --                                                                                 ##STR19##                                                                           ##STR20##                                                                          Spiro                                                                              0,034                                                                              139  4088                      7     3-Et--T                                                                               Eth. OH   CH3 --                                                                                 ##STR21##                                                                           ##STR22##                                                                          Spiro                                                                              0,073                                                                              73   1000                      8     3-All--T                                                                              All. OH   CH3 --                                                                                 ##STR23##                                                                           ##STR24##                                                                          Spiro                                                                              0,141                                                                              >200 >1418                     9     3-DMAll--T                                                                            DiMAll.                                                                             OH   CH3 --                                                                                 ##STR25##                                                                           ##STR26##                                                                          Spiro                                                                              0,239                                                                              73   252                       10    5-Et--U                                                                               --   OH   C2H5                                                                               --                                                                                 ##STR27##                                                                           ##STR28##                                                                          Spiro                                                                              0,038                                                                              3,2  82                        11    U      --   OH   H   --                                                                                 ##STR29##                                                                           ##STR30##                                                                          Spiro                                                                              0,114                                                                              8,3  73                        12    5-Br--U                                                                               --   OH   Br  --                                                                                 ##STR31##                                                                           ##STR32##                                                                          Spiro                                                                              0,206                                                                              2,35 11                        13    3-All--U                                                                              All. OH   H   --                                                                                 ##STR33##                                                                           ##STR34##                                                                          Spiro                                                                              0,364                                                                              5,31 15                        14    C      --   NH2  H   --                                                                                 ##STR35##                                                                           ##STR36##                                                                          Spiro                                                                              0,439                                                                              ≧200                                                                         ≧456               15    C      --   NHOAc                                                                               H   --                                                                                 ##STR37##                                                                           ##STR38##                                                                          Spiro                                                                              0,097                                                                              7,5  77                        16    5-Me--C                                                                               --   NH2  CH3 --                                                                                 ##STR39##                                                                           ##STR40##                                                                          Spiro                                                                              0,072                                                                              17,7 246                       17    A      --   --   --  NH2                                                                                ##STR41##                                                                           ##STR42##                                                                          Spiro                                                                              0,162                                                                              7,3  45                        __________________________________________________________________________      S.I. = Selectivity Index                                                 

                                      TABLE 2                                      __________________________________________________________________________     Inhibitory effects of substituted nucleoside dervatives according to the       present invention on                                                           the incorporation of [methyl-.sup.3 H]dThd, [5-.sup.3 H]dUrd, [5-.sup.3        H]dCyd, [5-.sup.3 H]Urd,                                                       [5-.sup.3 H]Cyd and L-[4,5-.sup.3 H]leucine into MT-4 cell DNA, RNA or         protein synthesis, respectively.                                               IC.sub.50.sup.a (μg/ml)                                                     Compound                                                                             [methyl-.sup.3 H]dThd                                                                   [5-.sup.3 H]dUrd                                                                     [5-.sup.3 H]dCyd                                                                     [5-.sup.3 H]Urd                                                                      [5-.sup.3 H]Cyd                                                                      L-[4,5-.sup.3 H]leucine                 __________________________________________________________________________      5    13       9.1   115   11    8.0   11.6                                     6    >100     >100  >100  >100  >100  >100                                     8    >100     >100  >100  >100  >100  >100                                     9    >100     >100  >100  >100  >100  >100                                    11    38       12    18    11    11    14                                      14    >100     >100  >100  >100  >100  >100                                    16    14       9.9   14    8.9   11    13                                      __________________________________________________________________________      .sup.1 50% Inhibitory concentration or compound concentration required to      inhibit incorporation of radiolabeled precursor.                          

We claim:
 1. A compound selected from the group consisting of the compounds graphically represented in formulas I and II, ##STR43## wherein B is a base selected from the group consisting of the formulas III, IV, V, VI, VII and VIII, ##STR44## wherein X₂ is selected from the group consisting of O, S and Se; wherein X₃ is selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl and benzyl;wherein X₄ is selected from the group consisting of OH, SH, NH₂, NHCH₃, N(CH₃)₂, and NHCOCH₃ ; wherein X₅ is selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, benzyl, halogen, cyano, thiocyano, hydroxymethyl, --CH₂ CH₂ -halogen, nitro and amino; wherein X₆ is selected from the group consisting of H, OH, halogen, NH₂, NHCH₃, and N(CH₃)₂ ; wherein X₇ is selected from the group consisting of hydrogen, alkyl and monomethoxytrityl; wherein X₈ is selected from the group consisting of halogen, amino, alkyl, alkenyl, alkynyl and mercapto; wherein X₉ is selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, amino and mercapto; wherein X₁₀ is selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, amino and mercapto; further wherein R₃, for formulas III-VIII is selected from the group consisting of formulas IX and X; ##STR45## wherein when R₃, has formula IX, R₁ is selected from the group consisting of hydrogen, alkyl, alkenyl and alkynyl; wherein when R₃, has formula X, R₁ is selected form the group consisting of hydrogen, amino, hydroxyl, carboxyl, mercapto, CH₂ NH₂, CONH₂, CH₂ OH, CH₂ NHOH, CH₂ SH, alkyl, alkenyl and alkynyl and R₂ is selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, amino and mercapto; and wherein R₅, and R₂, are selected from the group consisting of trimethylsilyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl, dimethylphenylsilyl, alkyl, aryl, and (CH₂)_(n) -phenyl with n=1-4.
 2. The compound of claim 1 wherein said compound is an N³ -alkylated 2', 5'-O-silylated-3'-spiro-5"-[4"-amino-1",2"-oxathiole-2",2"-dioxide] thymidine.
 3. The compound of claim 2 wherien said compound is {1-[2',5'-bis-O-(tert-butyldimethylsilyl)-β-D-ribofuranosyl]-3-N-methyl-thymine}-3'-spiro-5"-}4"-amino-1",2"-oxathiole-2",2"-dioxide} and has the formula ##STR46##
 4. The compound of claim 2 wherien said compound is {1-[2', 5'-bis-O-(tert-butyldimethylsilyl)-β-D-ribofuranosyl]-3-N-ethyl-thymine}-3'-spiro-5"-{4"-amino-1",2"-oxathiole-2",2"-dioxide} and has the formula ##STR47## 